The development of bioelectrochemical systems (BES) toward technological applications has increased over the past decade, leading to the development of numerous demonstration devices. Among these devices, microbial fuel cells (MFC) have gathered significant interest in both basic and applied research due to their potential to harness electrical current from a wide range of dissolved organic wastes or wastewaters and renewable biomass. This enables their use not only for sustainable energy production but also for bioremediation of contaminated environments, contributing for the development of the waste to energy technology that is a target of community research initiatives. (…) To explore the mechanisms by which Gram positive bacteria perform EET a multidisciplinary project will be conducted. In this project, the multiheme cytochromes involved in the extracellular electron transfer process of the Grampositive bacteria T.ferriacetica will be overexpressed and their structural and biochemical characterization will be carried out. This will be accomplished using a variety of spectroscopic techniques,(…).